MTA Regional Bus Operations (RBO) is the surface transit division of the Metropolitan Transportation Authority (MTA). It was created in 2008 to consolidate all bus operations in New York City operated by the MTA. As of February 2018, MTA Regional Bus Operations runs 234 local routes, 71 express routes, and 18 Select Bus Service routes. Its fleet of 5,725 buses is the largest municipal bus fleet in the United States and operates 24/7.

The division comprises two brands: MTA Bus and MTA New York City Bus. While MTA Bus is an amalgamation of former private companies' routes, MTA New York City Bus is composed of public routes that were taken over by the city before 2008. The MTA also operates paratransit services and formerly operated Long Island Bus.